Cloning and Expression Analysis of a Chalcone isomerase (CnCHI) Gene from Chamaemelum nobile
نویسندگان
چکیده
منابع مشابه
Cloning and Expression Analysis of ZmERD3 Gene From Zea mays
Background: Stresses (such as drought, salt, viruses, and others) seriously affect plant productivity. To cope with these threats, plants express a large number of genes, including several members of ERD (early responsive to dehydration) genes to synthesize and assemble adaptive molecules. But, the function of ERD3 gene hasn’t been known so far.Objectives:</strong...
متن کاملCloning, Expression Profiling and Functional Analysis of CnHMGS, a Gene Encoding 3-hydroxy-3-Methylglutaryl Coenzyme A Synthase from Chamaemelum nobile.
Roman chamomile (Chamaemelum nobile L.) is renowned for its production of essential oils, which major components are sesquiterpenoids. As the important enzyme in the sesquiterpenoid biosynthesis pathway, 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) catalyze the crucial step in the mevalonate pathway in plants. To isolate and identify the functional genes involved in the sesquiterpene b...
متن کاملcDNA cloning and expression analysis of the chalcone synthase gene (CHS) from Polygonum minus
Flavonoids are secondary metabolites that are present at high levels in the Polugonum minus. In recent years, flavonoids have attracted the interest of researchers because of their antioxidant properties .Flavonoids are synthesised via the condensation of 4 coumaryl-CoA and 3 malonyl-CoA by the action of Chalcone synthase. A cDNA encoding a chalcone synthase was isolated from the leaves of Poly...
متن کاملCloning and gene expression of cytochrome P450 gene from Alcanivorax borkumensis Bacterium
Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is...
متن کاملCloning, Expression and Purification of Chalcone Synthase from Solanum Tuberosum
Chalcone synthase (CHS) gene was amplified by PCR from cDNA of Solanum tuberosum leaf tissue. The gene was cloned into pGEMT easy vector and sequenced. It was then ligated into pET 30 vector to construct recombinant plasmid pET30-CHS. The recombinant was transformed into E.coli BL21 strain and the target gene was over expressed using IPTG. Optimum condition of IPTG concentration and temperature...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology(Faisalabad)
سال: 2017
ISSN: 1682-296X
DOI: 10.3923/biotech.2018.19.25